Supplementary Figure 6: Composition and stoichiometry of the TRF1-TIN2 and TRF1-TERB1 complexes.
From: Dissecting the telomere–inner nuclear membrane interface formed in meiosis
(a) Fluorescence-based competition experiments using Alexa Fluor 488-labeled GST-TIN2TBM pre-bound to biotin-tagged TRF1TRFH on streptavidin beads titrated with varying concentrations of unlabeled full-length TIN2 and TERB1TRFB – TERB21-107 proteins. Mean of technical duplicate is plotted. Error bars indicate s.e.m. (b) Size-exclusion (Superdex 200) profiles confirming intramolecular dimerization of TRFH-TRFH fusions (based on molecular weight determination). (c) Coomassie-stained SDS-PAGE for TRFH-TRFH intramolecular fusion proteins eluted by size-exclusion chromatography. (d) Replicate of experiment shown in Fig. 4c with quantitation shown at the bottom. (e) Mean and s.e.m. of duplicate experiments from panel d and Fig. 4c.
