Supplementary Figure 6: Analysis of the foot-containing 80S structure.
From: Preribosomes escaping from the nucleus are caught during translation by cytoplasmic quality control
(a) Magnification of the foot structure (PDB ID: 3JCT) fitted into the density of the 80S ribosome presented in this study. Factors belonging to the foot structure as well as ITS2 are highlighted in different colors and labeled accordingly. The structure of the 60S subunit (PDB ID: 5TGM) is fitted in order to display the main interactions between the foot structure and the 60S subunit. R-proteins interacting with the foot L8 (eL8), L25 (uL23) and L27 (eL27) are highlighted in green, yellow and red respectively (Wu, S. et al., Nature. 534, 133-7, 2016). 25S and 5.8S rRNAs are shown in gray and the rest of the r-proteins in beige. (b) The fit of the foot containing 80S ribosome into the density of actively translating polysomes (EMDB-2790) demonstrates that the foot (orange) would clash with the small subunit of the next ribosome (light blue). (c) The transition between the two conformations of ES27 (displayed in green and blue, PDB ID: 3IZF and 3IZD) may be obstructed by the presence of the foot.
