Supplementary Figure 5: The TERB1 647AEA649 mutant mice were generated using the CRISPR/Cas9 method.
From: Telomeric TERB1–TRF1 interaction is crucial for male meiosis
(a) The Cas9/sgRNA-targeting sites in mouse Terb1 gene. The sgRNA-targeting sequence and the protospacer-adjacent motif (PAM) are underlined. The desired mutations on genomic DNA are indicated by lower-case letters. (b) Transgenic mouse offspring were genotyped by tail biopsy with PCR and DNA sequencing of the mutation loci. (c) Relative mRNA expression was determined in testes of 3-week old littermates by RT-qPCR using GAPDH as the loading control. The averaged value for WT was normalized to 1. Results were from three independent experiments. Error bars: standard deviation; n=3. (d) Western blot of TERB1 in testes of two-week-old littermates. β-actin was used as the loading control. The indicated numbers below the blot images represent the relative signals of anti-TERB1 normalized by the signals of anti-Actin. Band intensity of blots was quantified by ImageJ. The number for WT sample was set as 1. Uncropped blot images are shown in Supplementary Data Set 1.
