Supplementary Figure 6: Hoechst 33342 fluorescence flow analysis of testicular cells from 6-week-old WT and Terb1AEA/AEA littermates.
From: Telomeric TERB1–TRF1 interaction is crucial for male meiosis
(a) Left panel: Schematic diagram summarizes the Hoechst 33342 fluorescent profile of male germinal cells in adult spermatogenesis. Right panel: Hoechst 33342 fluorescence flow analysis of testicular cells from 6-week-old WT and Terb1AEA/AEA littermates. L-Z: subpopulation that mainly comprises leptotene and zygotene spermatocytes. P-D: subpopulation that mainly comprises pachytene and diplotene spermatocytes. Subpopulation was sorted and analyzed by immunofluorescence staining of SYCP3 and γ-H2AX or propidium iodide staining to determine the cell types (data not shown). (b) Quantification of spermatid population in all testicular cells. Statistical significances (*** p<0.01) were assessed by two-tailed t-tests. Error bars: standard deviation; n=4. (c) Quantification of L-Z and P-D subpopulation in all spermatocyte I (4N DNA content). Statistical significances (** p<0.05) were assessed by two-tailed t-tests. Error bars: standard deviation; n=4.
