Supplementary Figure 4: Comparison of data collected in the presence of 500 μM dATP and 50 μM NTP on negatively and positively supercoiled templates. | Nature Structural & Molecular Biology

Supplementary Figure 4: Comparison of data collected in the presence of 500 μM dATP and 50 μM NTP on negatively and positively supercoiled templates.

From: TFIIH generates a six-base-pair open complex during RNAP II transcription initiation and start-site scanning

Supplementary Figure 4

The distributions of length changes for negatively supercoiled templates (green) and positively supercoiled templates (red) as compared to the DNA only control (black dashed). The changes in extension under different supercoiling directions are similar and in opposite directions suggesting that there is no large compaction occurring during DNA unwinding.

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