Supplementary Figure 5: Sub1 and TFIIA do not lead to larger bubbles.

Bubble size distributions in the presence (dotted line, N = 12) and absence of Sub1 and TFIIA. Between 28 and 100 nM Sub1 and 100 nM TFIIA were introduced to the flow cell concomitantly with the general transcription factors under conditions of 500 μM dATP and 50 μM NTP as described in the text.