Supplementary Figure 1: Separation and purification of lipid-free apoA-I monomer by gel filtration chromatography
From: A consensus model of human apolipoprotein A-I in its monomeric and lipid-free state
ApoA-I was cross-linked and subjected to gel-filtration chromatography and fractions corresponding to the stable monomeric species were pooled. Chromatograms of apoA-I cross-linked with CBDPS and BS3 are shown in panels (a) and (c), respectively. The shaded area represents the fractions corresponding to monomeric apoA-I that were pooled for cross-linking and SAXS analysis. Corresponding SDS-PAGE analysis of lipid-free apoA-I cross-linked with CBPDS and BS3 are shown in panels (b) and (d), respectively. Molecular weight markers are shown in lane 1, cross-linked apoA-I prior to separation is shown in lane 2, and cross-linked monomeric apoA-I after separation is shown in lane 3. Gels were stained with coomassie blue.
