Abstract
Piwi-interacting RNAs (piRNAs) are a novel class of small RNAs that are expressed specifically and abundantly in male germ cells. Here we report that the 3′ termini of piRNAs are 2′-O-methylated; this modification may have important implications for the biogenesis and function of piRNAs.
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References
Bartel, D.P. Cell 116, 281–297 (2004).
Carmell, M.A., Xuan, Z., Zhang, M.Q. & Hannon, G.J. Genes Dev. 16, 2733–2742 (2002).
Aravin, A. et al. Nature 442, 203–207 (2006).
Girard, A., Sachidanandam, R., Hannon, G.J. & Carmell, M.A. Nature 442, 199–202 (2006).
Grivna, S.T., Beyret, E., Wang, Z. & Lin, H. Genes Dev. 20, 1709–1714 (2006).
Lau, N.C. et al. Science 313, 363–367 (2006).
Watanabe, T. et al. Genes Dev. 20, 1732–1743 (2006).
Deng, W. & Lin, H. Dev. Cell 2, 819–830 (2002).
Kuramochi-Miyagawa, S. et al. Development 131, 839–849 (2004).
Elbashir, S.M., Lendeckel, W. & Tuschl, T. Genes Dev. 15, 188–200 (2001).
Hutvagner, G. et al. Science 293, 834–838 (2001).
Yang, Z., Ebright, Y.W., Yu, B. & Chen, X. Nucleic Acids Res. 34, 667–675 (2006).
Vagin, V.V. et al. Science 313, 320–324 (2006).
Pelisson, A., Sarot, E., Payen-Groschene, G. & Bucheton, A. J. Virol. (2006).
Keith, G. Biochimie 77, 142–144 (1995).
Ebhardt, H.A., Thi, E.P., Wang, M.B. & Unrau, P.J. Proc. Natl. Acad. Sci. USA 102, 13398–13403 (2005).
Silberklang, M., Gillum, A.M. & RajBhandary, U.L. Methods Enzymol. 59, 58–109 (1979).
Ohara, T. et al. Nat. Struct. Mol. Biol. advance online publication 25 March 2007 (doi:10.1038/nsmb1220).
Ma, J.B., Ye, K. & Patel, D.J. Nature 429, 318–322 (2004).
Acknowledgements
We thank T. Suzuki for communicating results of unpublished experiments and T. Suzuki and members of our lab for stimulating discussions. This work was supported by grants from US National Institutes of Health (GM0720777, NS053839 and P30-HD026979) and the Philadelphia Foundation to Z.M.
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Supplementary information
Supplementary Fig. 1
SYBR Gold staining of total mouse piRNAs. (PDF 228 kb)
Supplementary Fig. 2
Enzymatic sequencing of purified piR-1 and piR-1s. (PDF 570 kb)
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Kirino, Y., Mourelatos, Z. Mouse Piwi-interacting RNAs are 2′-O-methylated at their 3′ termini. Nat Struct Mol Biol 14, 347–348 (2007). https://doi.org/10.1038/nsmb1218
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DOI: https://doi.org/10.1038/nsmb1218
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