Figure 2: RRF orientation in the ribosome.
From: Crystal structure of the ribosome recycling factor bound to the ribosome
(a) Stereo view of unbiased difference Fourier maps (Fo − Fc) for RRF (orange backbone). Domain I is clearly visible, but the map is very noisy in the region where domain II is located. (b) Stereo view of difference Fourier maps calculated to 6 Å, in which helical parts of domain II become visible. (c) Overall orientation of RRF in the ribosome, with domain I spanning the 50S subunit's A and P sites. ASLPhe is in the 30S subunit's P-site, and tRNAfMet occupies the E site. Ribosomal proteins are shown in darker colors, and the mRNA is in magenta. (d) The tip of RRF would clash with a tRNA in the P/P state (modeled using the P-site ASLPhe) but would be compatible with a P/E hybrid state.
