Abstract
CLIP170 and p150Glued localize to the plus ends of growing microtubules. Using crystallography and NMR, we show that autoinhibitory interactions within CLIP170 use the same binding determinants as CLIP170's intermolecular interactions with p150Glued. These interactions have both similar and distinct features when compared with the p150Glued–EB1 complex. Our data thus demonstrate that regulation of microtubule dynamics by plus end–tracking proteins (+TIPs) occurs through direct competition between homologous binding interfaces.
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Acknowledgements
We thank M. Steinmetz and A. Akhmanova for sharing data before publication, R.C. Liddington and A. Wilde for critical reading of the manuscript and the staff of the Advanced Photon Source for assistance with data collection. This work was supported by grants from the National Cancer Institute of Canada. I.H. and M.J.P. acknowledge the Canadian Institutes of Health Research for postdoctoral fellowships. M.I. holds a Canada Research Chair in Cancer Structural Biology.
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Hayashi, I., Plevin, M. & Ikura, M. CLIP170 autoinhibition mimics intermolecular interactions with p150Glued or EB1. Nat Struct Mol Biol 14, 980–981 (2007). https://doi.org/10.1038/nsmb1299
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DOI: https://doi.org/10.1038/nsmb1299
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