Figure 3

Insulin Det attenuates weight and adipose gain in HFD, but not LFD rats. Body weight was measured daily in rats fed either LFD (a; n=5 per group) or HFD (b; n=6–8 per group) for each treatment group; Veh (circle), Det (triangle) and Glar (diamond). Body weight gain was determined for the 4-week study period for all groups (c). Fat mass was measured three times weekly by NMR in rats fed either LFD (d; n=5 per group) or HFD (e; n=6–8 per group) for each treatment group; Veh (circle), Det (triangle) and Glar (diamond). Fat mass gain was determined for the 4-week study period for all groups (f). Data are presented as the mean±s.e.m. and were analyzed by either two-way repeated measures ANOVA (for body weight and fat mass) or two-way ANOVA (for body weight and fat mass gain). Bonferroni's post-test shows that daily fat mass (e) was reduced by Det relative to Veh from 13 to 26 days and relative to Glar from 10 to 26 days. For all panels: ^*P<0.05, ^**P<0.01, and ^***P<0.001 for Veh vs Det; ^#P<0.05, ^αP<0.01 and ^βP<0.001 for Glar vs Det comparisons as analyzed by Bonferroni's post-test.