Figure 3

Hypothalamic ARC mRNA expression of orexigenic (upper panel) and anorexigenic (lower panel) neuropeptides in the JCR:LA-cp obese-prone and lean-prone rats following caloric restriction (n=6 rats per group). Data are shown as mean±s.e.m. *P<0.05 Lean-FF compared with Obese-FF; #P<0.05 Obese-FF compared with Obese-FR or Lean-FF vs Lean-FR. FF, free feeding; FR, food restricted. NPY, neuropeptide Y; POMC, pro-opiomelanocortin; CART, cocaine and amphetamine-regulated transcript; Mc3R, melanocortin receptor 3; Crhr1, corticotropin-releasing hormone receptor 1. Target gene expression was normalized to a reference gene (cyclophilin).