Figure 1

Effects of an LFD on Creb3l4 KO mice. Real-time PCR analysis of Creb3l4 mRNA levels in epididymal white adipose tissue (Epi-WAT) from db/db (a) and ob/ob (b) mice. The mRNA expression levels were normalized to those of Rplp0 (36B4) as a control. (c) Body weight and (d) increases in body weight of WT and Creb3l4 KO mice observed for 15 weeks on an LFD. (e) Weight and increased weight of Epi-WAT and liver in LFD-fed WT and Creb3l4 KO mice. (f) Hematoxylin and eosin (H&E) staining of subcutaneous white adipose tissue (Sub-WAT) and Epi-WAT from WT and Creb3l4 KO mice. (g) H&E stain of the liver and BAT. Scale bar, 500 μm. Values are expressed as means±s.e.ms., n=4–5 in panels (a–d), n=12–14 in panel (e), *P<0.05, **P<0.01.