Figure 3

Aged-Creb3l4 KO mice fed a chow diet show increased adiposity with impaired glucose tolerance and decreased insulin sensitivity. (a) Body weight changes in WT and Creb3l4 KO mice fed a chow diet for 84 weeks. (b) Body composition (% body weight) analysis of WT and Creb3l4 KO mice. (c) Hematoxylin and eosin (H&E) stain of subcutaneous white adipose tissue (Sub-WAT) and epididymal white adipose tissue (Epi-WAT) from aged-WT and aged-Creb3l4 KO mice (d) H&E stain of the liver and BAT from aged-WT and aged-Creb3l4 KO mice. Oral glucose tolerance test (OGTT) (e) and insulin tolerance test (ITT) (f) were performed after 90 weeks glucose intake and 92 weeks insulin adminstration, respectively. Values are expressed as means±s.e.ms., n=3–6 in panels (a, b, e and f). *P<0.05 for Creb3l4 KO versus WT.