Figure 1
From: Deletion of murine Arv1 results in a lean phenotype with increased energy expenditure
Generation of Arv1 knockout mice. (a) Predicted structure of the Arv1 protein showing the N-terminal Zn2+ binding domain, Arv1 homology domain (AHD), transmembrane domains (barrels) and luminal C-terminus. (b) Arv1 messenger RNA (mRNA) expression in tissues from male C57BL6/J mice 12–14 weeks of age (n=4), fold changes are shown relative to spleen, the lowest expression tissue. (c) Diagram of the murine Arv1 locus showing the locations of the PGK-neomycin resistance cassette (‘Neo’), LoxP sites (triangles), FRT sites (diamonds), Exons (closed boxes), AseI restriction sites (‘A’), and Southern blotting probe (probe). (d) Southern blot of wild-type (lane 1) and targeted (lane 2) embryonic stem cells. Digestion of the wild-type Arv1 allele with AseI produces a 7.2 kb fragment, while inclusion of the Neo cassette removes this AseI site and introduces a new site closer to exon 3, generating an 8.4 kb fragment. (e) Arv1 mRNA levels measured by real-time reverse transcriptase PCR (RT-PCR) of liver RNA isolated from wild-type (WT), heterozygous (Het) and homozygous null mice (KO) generated using CMV-Cre to delete Arv1 in the germline. Values are expressed as means±s.d. where the asterisk (*) indicates P<0.05 relative to the wild-type mice. CMV, cytomegalovirus.
