Figure 4
From: Lung cancer cell migration is regulated via repressing growth factor PTN/RPTP β/ζ signaling by menin
PTN increases interaction of RPTP β/ζ and integrins. (a) Serum-starved A549 cells were treated with 100 ng/ml recombinant human PTN (rhPTN) and harvested at various time points. RPTP β/ζ (red), β3 (green) and 4,6-diamidino-2-phenylindole (DAPI) (blue) were detected by IF, × 1600-fold. (b–d) A549 cell lysates were IP for RPTP β/ζ, αv and β3, and immunoprecipitates were analyzed by western blotting for the presence of RPTP β/ζ, αv, β3.
