Figure 5
From: Lung cancer cell migration is regulated via repressing growth factor PTN/RPTP β/ζ signaling by menin
Menin inhibits activation of FAK, PI3K and ERK1/2. (a) The efficiency of menin ablation and its effect on upregulation of pFAK, PI3K (85 kDa), pPI3K and pERK1/2 were determined by western blotting. (b) The effect of ectopic menin expression on FAK (125 kDa), pFAK, ERK1/2 (42 and 44 kDa) and pERK1/2 in A549 cells were determined using western blotting. (c) PAK1-PBD agarose and Rhotekin RBD agarose were used to isolate GTP-Cdc42, GTP-Rac1 and GTP-RhoA from whole cell lysates from menin-overexpressing A549 cells. The Cdc42-GTP, Rac1-GTP and RhoA-GTP were detected using western blotting and normalized by the total input protein. (d) Serum-starved A549 cells were treated with 100 ng/ml recombinant human PTN (rhPTN) and harvested at various time points. The activation of Cdc42, Rac1 and RhoA were detected by western blotting and normalized by the total input protein.
