Figure 8
Bid depletion inhibits TRAIL-induced apoptosis. (a) Expression of Bid analyzed by western blot in OVCAR3 cells incubated with transfectant only (vehicle), control siRNA (siRNALuc) for 48 h or Bid siRNA and cell lysates were obtained at 24 and 48 h after transfection. Bid expression was assessed by western blot. (b) Forty-eight hours after transfection, TRAIL (100 ng/ml) was added for 2 h, after which the number of colonies was determined by staining with crystal violet after 14 days. OVCAR3 cells were left untreated or treated with TRAIL (20 ng/ml) 48 h after the transfection with siRNAs for 48 h and apoptosis was measured by determining oligosomal DNA fragmentation 24 h after the addition of TRAIL (c) or by assessing the percentage of hypodiploid cells (d). Data shown are representative of three independent experiments. Values are the means±s.e.m. *P<0.001. (e) Bid expression in OVCAR3 cells transfected with siRNA as determined by western blot.
