Figure 2

Differential expression of genes in the mammary glands of MMTV-Cre Brca1^Co/Co mice at day 1 of lactation. (a) Experimental design. Gene expression profile analysis was performed on RNA extracted from the mammary glands of Brca1^Co/Co and MMTV-Cre Brca1^Co/Co mice at 10 weeks of age (virgin) and day 1 of lactation. Three pools of four individual mice were used to represent each stage and genotype. Data analysis revealed the indicated number of differentially expressed genes. (b) Results of ingenuity analysis showing the most significantly altered cellular and disease processes in MMTV-Cre Brca1^Co/Co mice. (c) Comparison of gene expression data in day 1 lactation MMTV-Cre Brca1^Co/Co mice compared with controls by microarray (fold change (white bars)) and validating quantitative real-time PCR (relative expression (grey bars)) of eight independently analysed individual mice. Error bars on microarray data represent s.e. of the three pools and on real-time PCR data represent the s.e.m. of eight biological replicates. Statistical analysis was performed using a Student's t-test with ***P<0.001 and **0.001<P<0.01. NS, not significant; qRT–PCR, quantitative real-time PCR.