Figure 5

c-kit does not regulate HC11 differentiation. Ectopic c-kit expression (a) and knockdown (b) was confirmed in HC11 cells by real-time PCR (left-hand graphs). c-kit mRNA levels were compared in HC11 cells transfected with (a) pBABE (control) and pBABE-c-kit or with (b) scrambled (control) and c-kit siRNA constructs. Bars represent the mean of three independent experiments (relative HPRT) and error bars represent s.e. Dome formation was unaffected by ectopic c-kit expression but was reduced with c-kit knockdown (right-hand graphs). Dome formation was calculated at 8 days after initiation of the assay and shown here relative to the pBABE (a) or scrambled (b) controls. Error bars represent s.e.