Figure 1
From: hTID-1 defines a novel regulator of c-Met Receptor signaling in renal cell carcinomas
hTid-1 modulates MetR tyrosine phosphorylation. (a) 786-0 parental RCC cells (786-0–VHL) and 786-0 cells in which VHL has been reintroduced (786-0+VHL) were infected with adenoviruses expressing either GFP alone (Ad-GFP), hTid-1S (Ad-hTid-1S) or hTid-1L (Ad-hTid-1L) as indicated. Cells were serum-starved overnight and either left untreated (−) or treated with 40ng/ml HGF (+). Whole-cell lysates were immunoblotted with antibodies specific to phospho-Met (Tyr1234/1235 in the activation loop; pMet), total Met, hTid-1, VHL and β-actin. Met antibodies detect Met molecules of 170 kDa (precursor) and 140 kDa (mature β-chain). (b) MDA-MB-231 cells were infected and treated as in panel a, except that cells were treated with HGF over the indicated time course. (c) Experiment was repeated as in panel b using A549 lung cancer cells.
