Figure 4
From: hTID-1 defines a novel regulator of c-Met Receptor signaling in renal cell carcinomas
hTid-1S enhances HGF-mediated migration. (a) hTid-1S (Ad-hTid-1S) and hTid-1L (Ad-hTid-1L) were overexpressed in 786-0 RCC cells via adenoviral infection. An adenovirus expressing GFP alone was used as a control (Ad-GFP). The cells were serum-starved overnight and plated in the upper chamber of a transwell chamber, using HGF as a chemoattractant in the lower chamber. After 24 h of incubation, the cells that had migrated were fixed and stained with Diff-Quik. Representative pictures from each filter are shown. (b) Lysates from cells infected simultaneously as those used for migration were immunoblotted with anti-hTid-1 to confirm efficient hTid-1 overexpression. (c) Migrated cells were counted in five individual fields and averaged. The results are pooled from three independent experiments. An unpaired t-test was used to determine significance between GFP and hTid-1S or hTid-1L overexpressing cells. *, a P-value of <0.05 was considered significant.
