Figure 5
From: hTID-1 defines a novel regulator of c-Met Receptor signaling in renal cell carcinomas
hTid-1S enhances HGF-mediated wound closure. (a) 786-0 RCC cells were infected with adenoviruses expressing either hTid-1S (Ad-hTid-1S) or hTid-1L (Ad-hTid-1L). A GFP expressing adenovirus (Ad-GFP) was used as a control. At 24-h post-infection, the cells were serum-starved for an additional 24 h. A uniform wound was made in the confluent cell layer at 0 h. The cells were then incubated with fresh serum-free media. (b) The same experiment was performed as shown in panel a, except that 5ng/ml HGF was added to the serum-free media at 0 h. Pictures of the wound at specific locations were taken at the indicated time points. (c, d) The wound width was calculated using Simple PCI software, and the relative wound closure was calculated setting wound closure of the control condition at 24 h as 1. The data presented is a representative experiment from three independent experiments. An unpaired t-test was used to calculate significance of either hTid-1S or hTid-1L overexpressing cells compared with the control at each time point. *, a P-value of <0.05 was considered significant.
