Figure 1 | Oncogene

Figure 1

From: PI3K inhibition results in enhanced HER signaling and acquired ERK dependency in HER2-overexpressing breast cancer

Figure 1

PI3K-pathway inhibition induces ERK phosphorylation in HER2-overexpressing cells. (a) Immunoblots of BT474 and MCF7-HER2 treated with BEZ235 (0–500 nM) for 24 h. (b) Immunoblots of MCF7-HER2 cells treated for 24 h with different PI3K-pathway inhibitors at the indicated concentrations (all in nM). (c) Immunoblots of p110-α knockdown MCF7-HER2 cells. SCR, scramble control; p110-α, siRNA for p110-α. Lapatinib treatment for 24 h. (d) Immunohistochemistry (IHC) of BT474-trastuzumab resistant (BT474-Tr) xenografts treated with BEZ235 (40 mg/kg, 4 × QD) with the indicated phospho-proteins. IHC pictures are representative of the 6-h time point.

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