Figure 3 | Oncogene

Figure 3

From: SPG20, a novel biomarker for early detection of colorectal cancer, encodes a regulator of cytokinesis

Figure 3

The SPG20-encoded protein Spartin is not expressed in hypermethylated colon cancer cell lines. (a) Western blot analysis showing levels of Spartin expression in colon epithelial cell lines. (b–g) Immunofluorescence confocal images of endogenous Spartin (red) in the indicated cell lines. The pictures in panels b and d–g are generated using identical microscopy settings for comparison of the Spartin level. The arrow points to a cytokinesis bridge (enlarged in panel c). The inset in panel c shows an overlay with phase-contrast image to visualize the midbody. Nuclei are shown in blue. The cells are fixed with 3% PFA. Size bars: 20 μm. PFA, paraformaldehyde; SPG20, spastic paraplegia-20.

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