Figure 4 | Oncogene

Figure 4

From: SPG20, a novel biomarker for early detection of colorectal cancer, encodes a regulator of cytokinesis

Figure 4

Spartin localizes to the spindle poles and the cytokinesis bridge of dividing cells. Three-dimensional reconstructions of immunofluorescence confocal images showing the localization of endogenous Spartin (red) to the cytokinesis bridge of HeLa cells (a–f) and to the spindle poles of prometaphase in HeLa (g, h, k) and CRL-1790 (i, j) cells. α-Tubulin (a–c, g–i) or γ-tubulin (j, k) is shown in green. Nuclei are shown in blue. Yellow indicates colocalization. The cells were pre-permeabilized with 0.05% saponin prior to fixation with 3% PFA (a–i) or fixed directly in methanol (j, k). Size bars: 5 μm. PFA, paraformaldehyde.

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