Figure 5
Colon cancer cell lines show several cytokinesis profiles. (a) The relative numbers of cells were quantified from immunofluorescence pictures using the Olympus Scan R microscope. (b) The cells were stained for α-tubulin and Aurora-B to visualize cytokinesis profiles. The immunofluorescence pictures were used to quantify the percentage of cells in cytokinesis. The error bars show the ±s.e.m. of four independent experiments. In total, more than 3000 cells were counted for the CRL-1790 control line, approximately 10 000 for HT29, and SW480 and between 30 000 and 40 000 for the HCT116 and RKO lines. (c–f) Three-dimensional reconstructions of immunofluorescence confocal z-stack images showing the difference in the amount of cytokinesis profiles between the four colon cancer cell lines (c, RKO; d, HCT116; e, SW480; f, HT29). Aurora-B (red), α-tubulin (green) and nuclei (blue). The arrow points to a cytokinesis profile. The cells were fixed with 3% PFA. PFA, paraformaldehyde.
