Figure 7
Rescue of Spartin expression results in the normalization of the cytokinesis process. Identical number of CRL-1790 cells (a–d) and HT29 cells (f–i) were seeded out on coverslips and treated or not with 1 μM AZA for 3 days as indicated in the figure. The cells were fixed with 3% PFA and stained for DNA (blue) and endogenous Spartin (red). (c, d, h, i) Magnified views of the areas indicated by white squares. (e, j) Western blots showing endogenous Spartin in AZA-treated versus that in non-treated cells. HT29 cells were treated (l) or not (k) with AZA, fixed with 3% PFA and stained for Aurora-B (red), α-tubulin (green) and DNA (blue). The difference in the number of cytokinesis profiles between AZA-treated and non-treated cells (arrowheads k, l) is shown in panel m. (n) Quantitations of relative growth rates. The error bars show the ±s.d. of four independent coverslips. For each condition, approximately 3000 cells were quantified for CRL-1790 and 30 000 cells for HT29. AZA, 5-aza-2′deoxycytidine; PFA, paraformaldehyde.
