Figure 3
CDDP induced calpain activation and enhanced its interaction and co-localization with p73α in OV2008, but not C13* cells. (a) CDDP induced calpain activation in OV2008, but not C13*. OV2008 and C13* cells were treated with different concentration of CDDP (0, 2.5, 5 and 10 μM; 24 h) and harvested at different times (0, 6, 12 and 24 h; 10 μM). Calpain activation was illustrated by cleaved α-fodrin (determined by western blot) (b) Exogenous TAp73α and ΔNp73α bind to calpain in control and CDDP-treated groups (c) Endogenous p73α and calpain interact in OV2008 cells. HA- TAp73α, HA-ΔNp73α and endogenous p73α were immunoprecipitated and then p73α isoforms and calpain binding was detected by western blot. (d) CDDP enhanced cytoplasmic p73α/calpain co-localization in OV2008 but not C13* cells. OV2008 and C13* cells were treated with CDDP (10 μM; 12 h) and p73α/calpain co-localization was detected by immunocytochemistry. Images represent three independent experiments.
