Figure 5
CDDP-induced [Ca2+]i increase depends upon internal stores. (a) CDDP-induced [Ca2+]i increase was not effected by the removal of extracellular Ca2+ (that is, Ca2+-free media). OV2008 cells were cultured in both regular and Ca2+-free media and the effect of CDDP on [Ca2+]i were measured. (b) CDDP-induced [Ca2+]i increase was ceased after store depletion by CPA in both the regular and the Ca2+-free media. OV2008 internal stores were depleted by CPA prior to CDDP treatment and changes in [Ca2+]i were assessed. (c) CDDP enhanced CPA-dependent calpain activation and apoptosis in OV2008, but not C13* cells. OV2008 and C13* cells were treated with CPA (100 μM; 24 h) and calpain activation, TAp73α and ΔNp73α content and apoptosis were determined. Images and figures present the mean±s.e.m. of three independent experiments (*P<0.05, ***P<0.001).
