Figure 3
From: PAX8 promotes tumor cell growth by transcriptionally regulating E2F1 and stabilizing RB protein
Depletion of PAX8 induces cell-cycle arrest through inhibition of DNA synthesis. (a) A498 and 786-O cells were transfected with the indicated siRNA. The cells were subjected to BrdU incorporation assays at 48 h post transfection (as described under Materials and methods). The percentage of cells in each cell-cycle phase is shown. (b) Experimental design for measuring S-phase progression using BrdU pulse–chase labeling assay. siRNA-transfected A498 and 786-O cells were pulse-labeled for 15 min with BrdU at 48 h post transfection. The S-phase cell population was monitored 24 h after pulse labeling. (c) FACS profiles for the indicated samples. Percentages of total cells with BrdU incorporated (R1, gray) and cells in S-phase (R2, black) were estimated. The data are the means±s.e.m. of three independent experiments. BrdU, 5-bromodeoxyuridine; siRNA, small interfering RNA.
