Figure 1
From: Hipk2 cooperates with p53 to suppress γ-ray radiation-induced mouse thymic lymphoma
LOH of Hipk2 in radiation-induced thymic lymphomas. (a) Detailed analysis of partial LOH patterns on chromosome 6 sublocalized three regions, denoted by the horizontal lines at the top of the figure. Physical mapping of markers and genes is based on the Ensembl database. Tumors showing either complete loss of chromosome 6 or no change are not shown in the Figure. LOH region 1 is centered on Hipk2. (b) mRNA expression of Hipk2 in thymic lymphomas examined by RT–PCR, showing that all lymphomas examined retained expression of the remaining allele of Hipk2 (c) Comparison of mRNA expression levels of Hipk2 in tumors with LOH versus those with retention of heterozygosity using a quantitative TaqMan assay. The average expression level in tumors with LOH (lanes 4–17) is reduced, compared with those without LOH (lanes 18–27), and with normal thymus (lanes 1–3).
