Figure 3
From: Hipk2 cooperates with p53 to suppress γ-ray radiation-induced mouse thymic lymphoma
Hipk2 is a haploinsufficient tumor suppressor gene. (a) LOH analysis of thymic lymphomas from Hipk2+/−p53+/− mice on chromosome 6 and 11. PCR analysis was carried out on 20 matched normal—tumor DNA samples to investigate loss of the wild-type alleles of Hipk2 (top panel) and p53 (bottom panel). Only one tumor (number 6) showed loss of the wild-type Hipk2 allele, but 18/20 showed loss of the wild-type p53 allele. (b) Expression analysis of Hipk2 in thymic lymphomas from Hipk2+/−p53+/− mice by RT–PCR analysis. Only tumor number6 that had lost the wild-type Hipk2 allele showed complete loss of Hipk2 expression.
