Figure 6

Distribution of identified candidate proteins in prostate cancer. (a–m) Low-magnification images (scale bar is 200 μm): all molecules showed stromal expression and ASPN, PARVA, CAV1, S100A6 and STC1 showed a localization to discrete areas of stroma and stromal subsets, while CTSK, FN, OGN, ZEB1 and CFH showed expression throughout the stroma. ASPN and STC1 showed staining in localized stromal areas containing tumour epithelia. (n–y) High-magnification images (scale bar is 50 μm): this confirmed that all candidates showed stromal staining, and also identified some epithelial staining for ASPN, ZEB1, NKTR and S100A6. Nuclear staining in epithelia was observed for ASPN and NKTR, while S100A6 was observed in some epithelia. Prostate cancer tissue was confirmed as tumour tissue by absence of epithelial microseminoprotein, beta (MSMB) expression (Supplementary Figure 5).