Figure 4
The methylation status of the rRNA promoter in prostate cancer cell lines and normal prostate epithelial cells. The methylation status for each cytosine within each CpG dinucleotide spanning from −158 to +29 bp of the human rRNA promoter in prostate cancer cell lines and normal prostate epithelial cells was determined. The rRNA promoter region was amplified from bisulfite-treated genomic DNA and cloned. The 10–18 randomly selected clones from each sample were subjected to automated sequencing.
