Table 3 Primers used in this study
Primer | Position | Sequence | Product Size (bp) |
|---|---|---|---|
45S rRNA forward | 851–868 | 5′-GAACGGTGGTGTGTCGTT-3′ | 130 |
45S rRNA reverse | 961–980 | 5′-GCGTCTCGTCTCGTCTCACT-3′ |  |
28S rRNA forward | 8352–8371 | 5′-AGAGGTAAACGGGTGGGGTC-3′ | 113 |
28S rRNA reverse | 8448–8464 | 5′-GGGGTCGGGAGGAACGG-3′ |  |
18S rRNA forward | 4026–4043 | 5′-GATGGTAGTCGCCGTGCC-3′ | 110 |
18S rRNA reverse | 4119–4135 | 5′-GCCTGCTGCCTTCCTTGG-3′ |  |
5.8S rRNA forward | 6642–6657 | 5′-ACTCGGCTCGTGCGTC-3′ | 136 |
5.8S rRNA reverse | 6762–6777 | 5′-GCGACGCTCAGACAGG-3′ |  |
TBP forward |  | 5′-CACGAACCACGGCACTGATT-3′ | 89 |
TBP reverse |  | 5′-TTTTCTTGCTGCCAGTCTGGAC-3′ |  |
rDNA genotyping forward |  | 5′-GTGTGTCCCGGTCGTAGG-3′ | 420 |
rDNA genotyping reverse |  | 5′-GTCACCGTGAGGCCAGAG-3′ |  |
rDNA promoter bisulfite sequence foward |  | 5′-GTTTTTGGGTTGATTAGA-3′ | 229 |
rDNA promoter bisulfite sequence reverse |  | 5′-AAAACCCAACCTCTCC-3′ |  |
rDNA promoter RTMSP forward |  | 5′-GGGGTTGATTAGAGGGTTTC-3′ | 189 |
rDNA promoter RTMSP reverse |  | 5′-CAACGTATCAACAATAACCCG A-3′ |  |
MYOD1 RTMSP forward |  | 5′-CCAACTCCAAATCCCCTCTCTAT-3′ | 162 |
MYOD1 RTMSP reverse |  | 5′-TGATTAATTTAGATTGGGTTTAGAGAAGGA-3′ |  |
mMYC forward |  | 5′-CAGATCAGCAACAACCGAAA-3′ | 168 |
mMYC reverse |  | 5′-GGCCTTTTCATTGTTTTCCA-3′ |  |
