Figure 7

Sequential GC/imatinib treatment causes apoptosis in imatinib-resistant CML CD34⁺ cells. (a) M1 cells were treated with dexamethasone for 24 h and then exposed to imatinib for 24 h. **P<0.001, Kruskal–Wallis test. (b) Same as in panel a, except that K562-r cells were used (*P<0.05, Kruskal–Wallis test). (c) Same as in panel a, but M2 cells were used. (d) WB analysis of P-AKT (Ser473), Bim and GILZ in M1 and CD34⁺ cells (Pt no. 4) treated with the indicated concentrations of dexamethasone for 24 h. (e) Same as in panel a, except that CD34⁺ cells from six imatinib-resistant patients were used. The graphs represent mean±s.d. of three separate experiments performed in triplicate.