Figure 3
From: Genome-wide mapping of Myc binding and gene regulation in serum-stimulated fibroblasts
The MDSR genes are enriched for direct Myc targets. (a) Quantitative RT–PCR validation of 45 MDSR genes. The expression of each mRNA at the time point of maximal activation by serum is shown in Myc-expressing cells (−OHT, blue bars) alongside Myc-deleted cells (+OHT, red bars). The data are the average±s.d. of three independent experiments. The asterisks mark the three genes that were not validated according to our criteria (see text). (b) Binding of Myc to the corresponding promoters (TSS±1 kb), as assayed by qChIP (top) and ChIP-seq (bottom). Cells were either starved for 2 days (blue bars) or stimulated with serum for 8 h (red bars) before harvesting.
