Figure 4
From: Genome-wide mapping of Myc binding and gene regulation in serum-stimulated fibroblasts
Distribution of Myc-binding peaks in the genome. (a) Examples of ChIP-seq profiles in serum-starved and stimulated cells are shown for two Myc-target loci, Serbp1 and Smyd2, aligned with CpG islands, E-boxes, the PCR amplicons used for validation and the corresponding qChIP profiles (bottom graphs). The qChIP data represent the average±s.d. from three independent experiments. (b) Relative frequency of peaks in the genomic window ±2 kb from all annotated TSSs, represented for cells without (black line) or with serum (red line). The vertical dotted line represents the mode of the distributions. (c) Box plots reporting the distribution of Myc-binding intensities (OCR-RMA) at peaks within ±1 kb from the TSS, shown separately for genes within each of the indicated regulatory patterns. Only genes bound above threshold were considered for each group (see text).
