Figure 1 | Oncogene

Figure 1

From: SirT3 suppresses hypoxia inducible factor 1α and tumor growth by inhibiting mitochondrial ROS production

Figure 1

SirT3 loss of function increases proliferation and HIF-1α activity. (a) Population doublings in primary SirT3 wild type (wt) and SirT3−/− (KO) MEFs cultured in normal oxygen conditions (21% O2). (b) Luciferase values from immortalized wt and KO MEFs transfected with HRE-luciferase in normoxia. SirT3 protein levels in 143B (c) and HCT116 (d) cells stably expressing a scrambled control shRNA (scr) or two different shRNAs that target SirT3. Both shRNA sequences increase the rate of cellular proliferation. Relative luciferase values of 143B (e) and HCT116 (f) cells in SirT3 stable knockdown cell lines (shRNA) or a scrambled control vector (scr) line transfected with HRE-luciferase. Error bars are s.e.m. and * indicates a P value <0.05 with two-tailed Student's t-test.

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