Figure 2
Knockdown of SirT3 augments the hypoxic response. (a) HIF-1α protein levels from whole cell lysates of indicated tumor cell lines incubated at normoxic (N, 21% O2) or hypoxic (H, 1% O2) conditions for 4 h. (b) Relative luciferase values of 143B cells in SirT3 stable knockdown cell lines (shRNA) or a scrambled control vector (scr) line transfected with HRE-luciferase cultured in normoxic (N, 21% O2) or hypoxic (H, 1% O2) conditions for 16 h. Quantitative PCR for VEGF-A (c), PGK-1 (d) and PDK-1 (e) on RNA isolated from 143B cells stably expressing SirT3 shRNA or a scrambled control vector (scr) cultured in normoxic (N, 21% O2) or hypoxic (H, 1% O2) conditions for 16hrs. Error bars are s.e.m. and * indicates a P value <0.05 with two-tailed Student's t-test.
