Figure 3
SirT3 gain of function inhibits hypoxic activation of HIF-1α. (a) Western blot of 143B cells stably overexpressing SirT3 tagged with V5. (b) Western blots of HIF-1α using total cell lysates from 143B control (c) and SirT3 overexpressing cells in normoxic (N, 21% O2) or hypoxic (H, 1% O2) conditions or treated with DMOG (d) for 16 h. (c) Relative luciferase values in 143B SirT3 overexpressing cells transfected with HRE-luciferase. (d) PGK1 mRNA from 143B SirT3 overexpressing cells in normoxic (N21% O2) or hypoxic (H, 1% O2) conditions. Error bars are s.e.m. and * indicates a P value <0.05 with two-tailed Student's t-test.
