Figure 4 | Oncogene

Figure 4

From: SirT3 suppresses hypoxia inducible factor 1α and tumor growth by inhibiting mitochondrial ROS production

Figure 4

ROS levels are regulated by SirT3. Relative levels of dihydroethidium (DHE) fluorescence in wild type (wt) or SirT3 −/− (KO) primary MEFs (a) and immortalized MEFs (b) treated with 10 μM DHE in normal oxygen conditions and then analyzed by flow cytometry. Each data point represents an independent culture of cells. (c) Relative fluorescence of 143B cells with SirT3 stably knocked down with two different SirT3 shRNAs or a scrambled control after incubation with DHE. (d) Western blots of whole cell lysates isolated from 143B cells stably overexpressing SirT3 Flag and their relative fluorescence after incubation with DHE with or without antimycin A (2 μg/ml). Error bars are s.e.m. and * indicates a P value <0.05 with two-tailed Student's t-test.

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