Figure 1
From: Silencing of Kruppel-like factor 2 by the histone methyltransferase EZH2 in human cancer
EZH2 depletion leads to increased KLF2 mRNA and protein levels. Expression of KLF2 and EZH2 determined by qRT–PCR (a) and (b) immunoblot in five cancer cell lines (U2OS, MDA-MB-231, MCF-7, PC3 and LNCaP cells) following transfection with oligo-type short interfering RNA (siRNA) against EZH2 or scrambled siRNA for 72 h as a transient model. (c) Immunoblot of EZH2 and KLF2 in stable EZH2 knockdown clones. U2OS cells are transfected with shRNA constructs targeting EZH2 or control vector and undergo selection with puromycin. P-values obtained from Student’s t-test.
