Figure 4
From: The HPV E6 oncoprotein targets histone methyltransferases for modulating specific gene transcription
E6 represses CRAM1 and PRMT1 activity-dependent p53 binding and histone modifications on p53-responsive promoters. U2OS cells transfected with the indicated expression vectors in the absence (lanes 1–6) or presence (lanes 7–9) of MG132 (a) and H1299 cells transfected with the indicated expression vectors in the presence of MG132 (b) were subjected to ChIP assays on the p21 or the GADD45 promoter, and western blotting was performed by using Abs against the indicated proteins. (c) HeLa cells treated with Adr and MG132 in the presence of scramble RNA (open square) or si-18E6 (filled square) were subjected to ChIP using Abs against the indicated proteins.
