Figure 3

Induction of aneuploidy results in cell transformation in vitro. (a) Anchorage-independent proliferation of the indicated cell lines on 1% agarose gel for 72 h was analyzed by using WST-1 and measuring absorbance at 450 nm (mean±s.e.m., n=4; ***P<0.05). (b) Proliferation of the indicated cell lines was analyzed in serum free medium using WST-1 (mean±s.e.m., n=4; ***P<0.001). (c) Apoptosis was scored using Apo-ONE reagent from the indicated cell lines with or without 24-h tumor necrosis factor-α (TNF-α) treatment (mean±s.e.m., n=4; ***P<0.001). (d) H-RasV12 or pBabe (control)-transfected cells were grown in soft agar for 23 days or allowed to form foci on plastic during 6 days. Graph shows quantification of foci number and representative images of foci and soft agar colonies from the indicated cells (mean±s.d, n=3; ***P<0.001). Numbers in the soft agar panels indicate mean number of very large colonies per well. A full colour version of this figure is available at the Oncogene journal online