Figure 4
β1YYFF_L4a cells have regained the ability to divide on β1-specific matrixes and traffic integrins. (a) Representative still images of time-lapse analysis of β1YYFF_L0a and β1YYFF_L4a cells undergoing cytokinesis on a β1-integrin-specific matrix. Graph shows quantification of cell division phenotypes (mean±s.e.m., 94–108 cells from three experiments, ***P=<0.0005). Numbers indicate minutes. (b) Representative micrographs and still images of time-lapse analysis of β1YYFF_L4a cells undergoing cytokinesis on a β1-integrin-specific matrix. Numbers indicate minutes. Cells were stained as indicated. (c) Biochemical analysis of endocytosis of cell surface biotinylated β1 integrin in β1YYFF_L4a and β1YYFF_L0a cells (mean±s.e.m., n=3). (d) The indicated cells were allowed to invade in growth factor reduced Matrigel for 4 days in the presence or absence of β1-function blocking antibody, after which the cells were fixed and stained with Alexa 546-phalloidin. Representative side view images of the invading cells, 50 μm grid. Numbers indicate the invasion area (pixels). All numerical data are representative from three or more independent experiments (mean±s.e.m., n=4; ***P<0.005).
