Figure 5 | Oncogene

Figure 5

From: A p27Kip1 mutant that does not inhibit CDK activity promotes centrosome amplification and micronucleation

Figure 5

p27K blocks the association of cyclin F with CP110. (a, b) HEK 293T cells were transfected with Flag-HA-cyclin F and either p27WT, p27K, p27CK or empty vector (pcDNA3, denoted by minus sign) using Fugene HD (Roche, Mannheim, Germany). Cells were harvested 48 h after transfection. (a) Anti-Flag immunoprecipitates were immunoblotted with antibody to CP110 or HA. Input was 0.75% of total protein used for immunoprecipitation. (b) Cell cycle position was determined by flow cytometry of propidium-stained cells.

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