Figure 3

Angiogenic genes expression is decreased in tumor tissue from p75KO mice. (a, b) Representative angiogenesis pathways microarray of tumors from WT and p75KO mice at 5 min exposure and the loading controls (bottom, small insets) at 5 s exposure time. Dotted-line circles indicate gene expression decrease in p75KO vs the same gene in WT (solid-line circles). (c) Functional grouping of genes in angiogenesis pathways microarray with the fold changes (dotted-line circles, decreased) in p75KO vs WT tumors. In p75KO tumor tissue decreased gene expression was observed in growth factors, cytokines and chemokines, such as vascular endothelial growth factor (VEGFA and B), placental growth factor (PGF), chemokine (C-X-C motif) ligands (Cxcl1, Cxcl2, Cxcl10), interleukins (IL1b, IL12a and IL18), pleiotrophin (Ptn, known as, heparin-binding growth factor 8), signal transduction and transcription factors MAPK14 (known as p38), prostaglandin-endoperoxide synthase 1 (Ptgs1), adhesion molecules and proteases, such as collagen, type XVIII, alpha 1 (Col18a1), neuropilin 1 (Nrp1), angiopoietin-like 4 (Angptl4) and matrix metalloproteinase 9 (Mmp 9). The densitometry values of Hsp90ab1 and BAS2C genes were used as internal control. An arbitrary cutoff was set at 1.6 (up or down). We assigned an arbitrary cutoff thresholds for fold changes at 1.6 (here and elsewhere).