Figure 6
From: Breaking the ‘harmony’ of TNF-α signaling for cancer treatment
Percent inhibition of p75 receptor expression correlates directly with increase in TNF-mediated apoptosis and decrease in angiogenic gene expression in p75KD/LLCs. (a) Representative histograms of FACS analysis of propidium iodide (PI)-stained two p75KD/LLC (transfected with p75 shRNA plasmid combinations 1+2 and 1−4), where M1 gates represent sub-G0/G1 population of the cells with less than 2n DNA, presumably, apoptotic cells. (b–e) Constitutive and TNF-mediated (80 ng/ml) expression (qRT–PCR analysis) for various angiogenic genes—(b) VEGFA, (c) HGF, (d) PLGF, and (e) CXCR4 for the same two p75KD/LLCs 24 h post-TNF treatment.
